| Place | Bib Number | Name | Affiliation | Time |
| 1 | 389 | Allie Clukey | Bangor High School | 26.58 |
| 2 | 642 | Rachael Mumm | Scarborough High School | 26.67 |
| 3 | 241 | Kelsey Karkos | Mt. Ararat High School | 27.02 |
| 4 | 498 | Kelsey Pettengill | Edward Little High School | 27.41 |
| 5 | 312 | Tess Perry | Mt. Blue High School | 27.69 |
| 6 | 345 | Lindsey Alston | Cheverus High School | 27.80 |